Prostaglandin D2 receptor expression in the lung and the effect of PPAR-γ, DP1, and DP2 receptor agonist treatment during respiratory viral infection

Abstract Prostaglandin D2 (PGD2) may play an anti-inflammatory role in the lung during respiratory viral infection, but little is known about expression of its receptors (PPAR-γ, DP1, and DP2) or mechanism action. Adult C57BL/6 mice were infected with Sendai virus (500 pfu /g body weight), a natural mouse pathogen that infects airway epithelial cells. Immunostaining uninfected lungs showed PPAR-γ protein cells larger airways, not alveolar After was no longer detectable lungs. Immunofluorescence DP1 present parenchymal as well some CCSP+ cells, levels did appear to change infection. DP2 increased after infection predominantly 24 hours post-infection. also co-localized MHC Class II+ parenchyma on days 3 7 Viral primary tracheal mRNA 48 72 There vitro. To test effect each receptor vivo, treated PPAR-γ, agonists, vehicle every 12 hours. Mice agonist protected from weight loss had reduced IFN-γ day 7, suggesting PGD2 be acting via reduce inflammation lung. Supported by grants Sigma Xi Grant Aid Research Saint Louis University College Arts Sciences Brennan Award.